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The reaction was carried out in 1 mL final volume, which contained 0.75 mL of Tris buffer (111 mM, pH 7.5), 0.05 mL of NADH (1.8 mM) and 0.1 mL of cytosol

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Typically, diazepam 10–20 mg or chlordiazepoxide 100 mg doses are repeated every 1–2 h until the patient reaches adequate sedation with an average of three doses usually required.3 Studies found diazepam loading to significantly reduce the risk of complications, to reduce the total dose of benzodiazepines needed, and the duration of withdrawal symptoms