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The membrane-bound NOXs enzymes, which under normal circumstances are dormant, may be a significant source of ROS upon stimulation by a variety of triggers including growth factors, proinflammatory cytokines, infections, and hormones [48]
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To confirm this finding, we carried out ChIP-qPCR using anti BRD4 and anti H3K4me3 antibodies in acute myeloid leukemia Kasumi-1 and chronic myelogenous leukemia K562 cell lines, characterized by the presence of endogenous ASXL1 1-645 and ASXL1 1-591 truncation variants, respectively
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