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The DNA fragment encoding U6atac snRNA was made from synthetic oligos (U6atac 1/2/3F & 1/2/3R), with the following amplification using primer pair BsmBI-U6F/U6R-BamHI and cloned into BsmBI/BamHI sites of lentiviral plasmid lenti sgRNA(MS2)_puro backbone (Addgene, #73795) to generate lenti-U6atac plasmid
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