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Plasma protein extraction and digestion For the discovery cohort (2011), two separate preparations were made with either 1 or 2 ยตL of plasma added to new tubes containing 24โ23 ยตL of SDC buffer consisting of sodium deoxycholate 1% (DOC), tris(2-carboxyethyl) phosphine 10 mM (TCEP), chloroacetamide 40 mM and Tris 100 mM pH 8.5
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Patch testing is recommended before initiating treatment, especially for patients with histories of metal sensitivities
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