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To determine if APAP ingestion resulted in increased ROS in the Drosophila fat body, we stained APAP-treated and control early third-instar larvae with HydroCy3, a ROS sensitive dye that fluoresces in the presence of superoxide radicals 29
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Melatonin, for instance, decreases lipid peroxidation, boosts antioxidant levels, and inhibits caspase-3 activity, all of which maintain hepatocyte integrity (Elsawy et al
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