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Monitoring glucose uptake in C2C12 and HepG2 cells where the expression of DNAJB3 has been silenced was done by transfecting cells with DNAJB3-siRNA or scrambled control and after 2 days, cells were pre-treated with either 0.3 mM ALA or vehicle for 8 h and the media was changed to glucose free media with either ALA or vehicle for starvation and glucose uptake was carried out as described above
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Pharmacological agents such as fenofibrate, carnosine, umbelliferone, paricalcitol, leonurine, triptolide, DDO-1039, and Potentilla Discolor Bunge(whose main active components are quercetin, kaempferol, and β-sitosterol) can upregulate NRF2, thereby suppressing ferroptosis and decelerating the progression of diabetic nephropathy [118,119,120,121,122,123,124,125,126]
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