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Inhibition of these target enzymes, which include both intracellular and extracellular targets, results in (inter alia) pyroptosis of tumor- associated macrophages, and the release of IL-lbeta and perhaps other immunostimulatory cytokines, and the effects of treatment with an immuno-DASH inhibitor include redistribution and altered activity of tumor associated MDSCs, enhanced priming of T- cells and dendritic cells, and enhanced trafficking of T-cells and other immune cells to the tumor

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The absorbance of the resulting solution was measured at 490 nm using the phenol-sulfuric acid colorimetric method