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Respective HRP-conjugated secondary antibodies were diluted in 0.5% milk in TBST and incubated with the membranes for 1 h

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Figure 1 3 ROS-mediated signaling pathway The physiological consequences of oxidative stress primarily stem from its capacity to modulate diverse signaling pathways, including the nuclear factor κB (NF-κB), mitogen-activated protein kinase (MAPK), Kelch-like ECH-associated protein 1/nuclear factor E2-related factor 2 (Keap1/Nrf2), and AMP-activated protein kinase (AMPK) pathways