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The fact that the AcpS F50/50A mutant was enzymatically active allowed us to determine if the IC 50 against DNM0547 increased as would be predicted if AcpS F50/50 was important for DNM0547 binding, we used the AcpS N82/81A and AcpS H-/108A as controls as they lie within the active site and were not predicted to contribute to DNM compound binding

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Thus, the GSH content of the liver can be quickly depleted in ∼1 day with fasting and starvation