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3.5 gsRBD induced the production of broad neutralizing antibodies against Beta, Delta, and Omicron variants Since the outbreak, multiple variants of SARS-CoV-2 have emerged ( Firstly, we measured the titers of IgG antibodies against the RBDs of the Beta (B.1.351), Delta (B.1.617.2), and Omicron (B.1.1.529) variants using the Day 49 sera from mice immunized with gsRBD and wtRBD in combination with the Freund’s adjuvant ( Figures 4A-C , Supplementary Figures 7A-C )