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Cells were then washed with binding buffer, and 20 ΞΌL aliquots were mounted on glass slides and examined using a fluorescence microscope (EVOS FL, Thermo Fisher Scientific)
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5.2 Nrf2 / HO-1 pathway The Nrf2 pathway has been proven to be a therapeutic pathway associated with UC and can inhibit ferroptosis and protect IECs through the Nrf2 /GPx4 signaling pathway ( Nrf2 is a key factor in inhibiting ferroptosis, yet excessive activation might lead to ferroptosis
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