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GSH's free thiol (pKa ~9) acts as a reductant: glutathione peroxidase uses two GSH molecules to reduce H₂O₂ or lipid peroxides (producing GSSG and water/alcohol), and NADPH-dependent glutathione reductase regenerates the reduced form
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PEG-catalase efficiently protected the cells against TAM by removing hydrogen peroxide and total ROS, whereas a less protective effect was observed in cells pretreated with PEG-SOD to eliminate O 2 •− anion (Fig
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