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Activated HSCs proliferate and secrete ECM components, including collagen and fibronectin, in response to inflammatory mediators released by damaged hepatocytes and liver cells, such as Kupffer cells [12]
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6 µM rabbit GAPDH (Sigma-Aldrich) was incubated for 20 min in a total volume of 500 µl of 100 mM Tris/HCl p H 8.0, 1 mM EDTA, 1.4 mM H 2 O 2 and 3 mM GSH, which led to glutathionylation and inactivation of enzymatic activity
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