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This review summarizes our recent work on AAT focusing on: 1) the protonation state of PLP in the active site and how it influences reaction specificity and catalytic power, 2) the magnitude of ground state destabilization in the external aldimine intermediate, 3) the enhancement of the catalytic activity of free and AAT bound PLP by light, and 4) the interconversion of the substrate specificity of AAT and tyrosine aminotransferase (TAT) by bioinformatics methods we have developed
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