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Step-by-Step Injection Protocol Preparation : Remove reconstituted Epithalon from refrigerator 10-15 minutes before injection to reach room temperature, reducing injection discomfort [3] Hand hygiene : Wash hands thoroughly with soap and water, allow to air dry completely Site selection : Choose injection site on abdomen (2 inches from navel), outer thigh (middle third), or upper arm (posterior aspect) [4] Site preparation : Clean injection site with 70% isopropyl alcohol using circular motion, allow to dry 30 seconds Syringe preparation : Draw prescribed dose using aseptic technique, expel air bubbles by gently tapping syringe and pushing plunger Injection angle : Insert needle at 45-degree angle for patients with adequate subcutaneous tissue, or 90-degree angle for lean individuals [4] Injection technique : Pinch skin fold, insert needle completely, inject slowly over 5-10 seconds to minimize tissue trauma Post-injection : Remove needle quickly, apply gentle pressure with alcohol pad for 10-15 seconds, do not massage injection site Disposal : Dispose of used syringe and needle in appropriate sharps container immediately Documentation : Record injection site, dose, time, and any reactions in treatment log Injection Site Rotation Schedule Proper injection site rotation prevents lipodystrophy and maintains consistent absorption rates throughout treatment cycles [4]