nadph and glutathione levels u87 cells Pro-Oxidant Auranofin Glutathione-Depleting Combination Unveils Synergistic Lethality in Glioblastoma with Aberrant Epidermal Growth Factor Receptor Expression Pack Size:10 Pack

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Realistic expectation: Weeks 4-6 represent the first meaningful assessment window

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Cells were then treated (duplicate wells/treatment) with stuarosporine (1 μM) for 24 h [21] or H 2 O 2 (100–2500 μM, Sigma-Aldrich) for 2 h at 37 °C ± a 30 min pre-treatment with inhibitors of cell death: liproxstatin (LPX, 10 µM, Sigma-Aldrich) and necrostatin-1 (25 µM) [22]